Channelrhodopsin2 Current During the Action Potential: “Optical AP Clamp” and Approximation
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Channelrhodopsin2 Current During the Action Potential: “Optical AP Clamp” and Approximation
The most widely used optogenetic tool, Channelrhodopsin2 (ChR2), is both light- and voltage-sensitive. A light-triggered action potential or light-driven perturbations of ongoing electrical activity provide instant voltage feedback, shaping ChR2 current. Therefore, depending on the cell type and the light pulse duration, the typically reported voltage-clamp-measured ChR2 current traces are ofte...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2014
ISSN: 2045-2322
DOI: 10.1038/srep05838